Christina Laun from Virtual Hosting dot com has written an article looking at web design guideline based on eye momvement research studies. She has listed 23 guidelines (see Christina’s full post here)

1. Text attracts attention before graphics.
2. Initial eye movement focuses on the upper left corner of the page.
3. Users initially look at the top left and upper portion of the page before moving down and to the right.
4. Readers ignore banners.
5. Fancy formatting and fonts are ignored.
6. Show numbers as numerals.
7. Type size influences viewing behavior.
8. Users only look at a sub headline if it interests them.
9. People generally scan lower portions of the page.
10. Shorter paragraphs perform better than long ones.
11. One-column formats perform better in eye-fixation than multi-column formats.
12. Ads in the top and left portions of a page will receive the most eye fixation.
13. Ads placed next to the best content are seen more often.
14. Text ads were viewed mostly intently of all types tested.
15. Bigger images get more attention.
16. Clean, clear faces in images attract more eye fixation.
17. Headings draw the eye.
18. Users spend a lot of time looking at buttons and menus.
19. Lists hold reader attention longer.
20. Large blocks of text are avoided.
21. Formatting can draw attention.
22. White space is good.
23. Navigation tools work better when placed at the top of the page.